Mol Biol & Biochem Protocols

Molecular Biology and Biochemistry Protocols Package v1.0

* Over 70 most common protocols and buffer recipes used in the Molecular Biology, Biochemistry and Cell Biology labs.

* NEW: The protocols can be edited in Word and saved.

* The program can be copied to SD card.

* No internet connection is required to use the program (100% offline).

* The package will continue to grow and regular updates will be provided for free.

* If you would like to add any protocol to this package, please email your suggestion/protocol to info@proteinguru.com

* Note: The package can be uninstalled and refunded within the first 15 minutes of purchase (Android Market rules).

Table of Contents for v1.0 (Jan 2012 release):

DNA World:
Agarose gel electrophoresis and PCR Check
Colony PCR from yeast colonies (application: Y2H screens)
Touch-down PCR protocol
10 Rules of Primer Design
Restriction Digestion Setup
Qiagen’s PCR purification column
Ligation Reaction Setup
T-vector for cloning PCR products
Phenol-Chloroform DNA isolation
Yeast Transformation
CIP-treatment
Plasmid Prep (classical protocol)
Miniprep (Qiagen kit)
Silver Staining of DNA Gels
LB-Ampicillin Plates
Chemically Competent E. coli Preparation
Transformation of the Chemically Competent E. coli Cells
Transformation - 5min Shortcut for Ampicillin Resistant Plasmids
Chromatin Immunoprecipitation (ChIP) Protocol

RNA World:
siRNA resuspension
RNA isolation from cultured cells by using RNeasy kit (Qiagen)
RT-PCR with oligo(dT) primers

Protein World:
General IP protocol
Protein expression in E. coli.
Purification of His-tagged proteins from E. coli
SDS-PAGE
Coomassie Brilliant Blue Staining of Protein Gels
Silver Staining of Protein Gels
Western Blotting
Western Blot Stripping
Preparation of Enhanced ChemiLuminescence (ECL) Substrate Buffers
IP-kinase protocol for P81 paper assay
IP-kinase protocol for autoradiography
Preparation of SILAC media
Sample Preparation for phospho-SILAC (global phosphoproteomic analysis)
Phospho-SILAC Analysis using MAXQUANT

Cell World:
Freezing cells
Hoechst staining
Immunofluorescence
Propidium Iodide Staining of Cells for Cell Cycle Analysis by FACS
Transfection of cells using PEI

C. elegans World:
Egg prep protocol
Myosin purification from C. Elegans

Buffer and Solution Recipes:
PBS
TBS
TBST
5X SDS-PAGE Sample Buffer
6X Agarose Loading Buffer
40% acryl stock (29:1) (for DNA gels)
20% paraformaldehyde stock
0.5M ATP stock
1M DTT
0.5M EDTA
50X TAE
10X TBE
TE
20X SSC
LB medium
SOC medium
M9 Buffer
Lysis / IP Buffer